Hotspot

ndhC-trnV-UAC

LSC · 50037-50719 · 683 bp

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Pi 0.0296
MarkerSeek score 66.10
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
683
variable_sites
54
pi
0.0296
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TTTCTCTGGCCCCTTCCTAA GGTTCGAATCCGTATAGCCC 50.0 55.0 57.9 57.9 825-1026 bp 1.0 85.6
ndhC-trnV-UAC_p2 TTTCTCTGGCCCCTTCCTAA CAGGTCTACGGTTCGAATCC 50.0 55.0 57.9 57.8 834-1035 bp 1.0 84.5
ndhC-trnV-UAC_p3 TTTCTCTGGCCCCTTCCTAA CGGTTCGAATCCGTATAGCC 50.0 55.0 57.9 58.3 826-1027 bp 1.0 83.8
ndhC-trnV-UAC_p4 TCTCTGGCCCCTTCCTAATT GGTTCGAATCCGTATAGCCC 50.0 55.0 57.7 57.9 823-1024 bp 1.0 83.8
ndhC-trnV-UAC_p5 TTCTCTGGCCCCTTCCTAAT GGTTCGAATCCGTATAGCCC 50.0 55.0 57.7 57.9 824-1025 bp 1.0 83.8