Hotspot

trnT-UGU-trnL-UAA

LSC · 46184-46944 · 761 bp

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Pi 0.0182
MarkerSeek score 60.50
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
761
variable_sites
39
pi
0.0182
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG CCGTAGCGTCTACCAATTCC 55.0 55.0 57.9 58.2 675-823 bp 1.0 85.7
trnT-UGU-trnL-UAA_p2 GCGATGCTCTAACCTCTGAG AGTCCGTAGCGTCTACCAAT 55.0 50.0 57.9 58.2 678-826 bp 1.0 84.9
trnT-UGU-trnL-UAA_p3 GGAATCGAACCGATGACCAT CCGTAGCGTCTACCAATTCC 50.0 55.0 57.8 58.2 707-855 bp 1.0 84.7
trnT-UGU-trnL-UAA_p4 GGAATCGAACCGATGACCAT AGTCCGTAGCGTCTACCAAT 50.0 50.0 57.8 58.2 710-858 bp 1.0 83.9
trnT-UGU-trnL-UAA_p5 GCGATGCTCTAACCTCTGAG TAAGTCCGTAGCGTCTACCA 55.0 50.0 57.9 57.6 680-828 bp 1.0 83.6