Hotspot

trnE-trnT-GGU

LSC · 30649-31470 · 822 bp

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Pi 0.0219
MarkerSeek score 65.20
Primer available yes
Label trnE-trnT-GGU

Feature Metadata

label_name
trnE-trnT-GGU
feature_type
igs
region
LSC
length_bp
822
variable_sites
51
pi
0.0219
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-trnT-GGU_p1 GCTGCCTCCTTGAAAGAGAG GAACCGATGACTTACGCCTT 55.0 50.0 58.3 58.0 899-929 bp 1.0 86.3
trnE-trnT-GGU_p2 TGTCCTGAACCACTAGACGA GAACCGATGACTTACGCCTT 50.0 50.0 57.7 58.0 878-908 bp 1.0 86.2
trnE-trnT-GGU_p3 GCTGCCTCCTTGAAAGAGAG CCATGGCGTTACTCTACCAC 55.0 55.0 58.3 58.1 878-908 bp 1.0 85.9
trnE-trnT-GGU_p4 TGTCCTGAACCACTAGACGA CCATGGCGTTACTCTACCAC 50.0 55.0 57.7 58.1 857-887 bp 1.0 85.9
trnE-trnT-GGU_p5 GCTGCCTCCTTGAAAGAGAG TGGCGTTACTCTACCACTGA 55.0 50.0 58.3 58.1 875-905 bp 1.0 85.8