Hotspot

trnS-GCU-trnT-CGU

LSC · 8086-8753 · 668 bp

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Pi 0.0279
MarkerSeek score 72.80
Primer available yes
Label trnS-GCU-trnT-CGU

Feature Metadata

label_name
trnS-GCU-trnT-CGU
feature_type
igs
region
LSC
length_bp
668
variable_sites
56
pi
0.0279
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnT-CGU_p1 CCAACGCTTTAGTCCACTCA AGGGGTTAAAGGAACGAATCA 50.0 42.9 57.8 56.9 691-840 bp 1.0 72.0
trnS-GCU-trnT-CGU_p2 CCAACGCTTTAGTCCACTCA GGGGTTAAAGGAACGAATCAC 50.0 47.6 57.8 56.9 690-839 bp 1.0 71.8
trnS-GCU-trnT-CGU_p3 AACGCTTTAGTCCACTCAGC AGGGGTTAAAGGAACGAATCA 50.0 42.9 58.2 56.9 689-838 bp 1.0 71.7
trnS-GCU-trnT-CGU_p4 AACGCTTTAGTCCACTCAGC GGGGTTAAAGGAACGAATCAC 50.0 47.6 58.2 56.9 688-837 bp 1.0 71.5
trnS-GCU-trnT-CGU_p5 CCAACGCTTTAGTCCACTCA AGGGGTTAAAGGAACGAATCAC 50.0 45.5 57.8 58.3 691-840 bp 1.0 70.1