Hotspot

psbI-trnS-GCU

LSC · 7913-7997 · 85 bp

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Pi 0.0261
MarkerSeek score 59.90
Primer available yes
Label psbI-trnS-GCU

Feature Metadata

label_name
psbI-trnS-GCU
feature_type
igs
region
LSC
length_bp
85
variable_sites
7
pi
0.0261
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-trnS-GCU_p1 TCCTATCTAATGATCCAGGACGT TAGGAGAGATGGCTGAGTGG 43.5 55.0 58.0 57.9 209-224 bp 1.0 61.6
psbI-trnS-GCU_p2 TCCTATCTAATGATCCAGGACGT TGAGTGGACTAAAGCGTTGG 43.5 50.0 58.0 57.8 196-211 bp 1.0 61.3
psbI-trnS-GCU_p3 TCCTATCTAATGATCCAGGACGT GCTGAGTGGACTAAAGCGTT 43.5 50.0 58.0 58.2 198-213 bp 1.0 61.0
psbI-trnS-GCU_p4 TCCTATCTAATGATCCAGGACGT CGAGTTAATCGTACCGAGGG 43.5 55.0 58.0 57.6 159-174 bp 1.0 60.2
psbI-trnS-GCU_p5 TCCTATCTAATGATCCAGGACGT ATGGCTGAGTGGACTAAAGC 43.5 50.0 58.0 57.6 201-216 bp 1.0 59.2