Hotspot

trnH-GUG-psbA

LSC · 77-644 · 568 bp

Back to Isatis

Pi 0.0936
MarkerSeek score 73.00
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
568
variable_sites
122
pi
0.0936
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.88
markerseek_score
73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 CTGCCTTAATCCACTTGGCT GCTGTTGAGGCTCCATCTAC 50.0 55.0 57.9 58.1 341-673 bp 1.0 90.5
trnH-GUG-psbA_p2 CTGCCTTAATCCACTTGGCT CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 356-688 bp 1.0 90.4
trnH-GUG-psbA_p3 CTGCCTTAATCCACTTGGCT TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 359-691 bp 1.0 90.3
trnH-GUG-psbA_p4 ACTGCCTTAATCCACTTGGC GCTGTTGAGGCTCCATCTAC 50.0 55.0 58.2 58.1 342-674 bp 1.0 90.3
trnH-GUG-psbA_p5 ACTGCCTTAATCCACTTGGC CCTCTAGACCTAGCTGCTGT 50.0 55.0 58.2 57.9 357-689 bp 1.0 90.2