Hotspot

petG-trnW-CCA

LSC · 64463-64588 · 126 bp

Back to Isachne

Pi 0.0246

MarkerSeek score 69.60

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 126
variable_sites: 3
pi: 0.0246
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	GGGATTATTCGTGACTGCGT	CAAAACCCGATGTCGTAGGT	50.0	50.0	58.1	57.9	219-223 bp	1.0	81.3
petG-trnW-CCA_p2	AGCGGGATTATTCGTGACTG	CAAAACCCGATGTCGTAGGT	50.0	50.0	57.8	57.9	222-226 bp	1.0	80.0
petG-trnW-CCA_p3	GCGTATTTGCAATACAGGCG	CAAAACCCGATGTCGTAGGT	50.0	50.0	58.3	57.9	203-207 bp	1.0	80.0
petG-trnW-CCA_p4	GGGATTATTCGTGACTGCGT	CCCGATGTCGTAGGTTCAAA	50.0	50.0	58.1	57.6	214-218 bp	1.0	79.1
petG-trnW-CCA_p5	GGGATTATTCGTGACTGCGT	TCCAAAACCCGATGTCGTAG	50.0	50.0	58.1	57.6	221-225 bp	1.0	79.0