Hotspot

rbcL-psaI

LSC · 56988-57907 · 920 bp

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Pi 0.0164

MarkerSeek score 55.80

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 920
variable_sites: 15
pi: 0.0164
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	GAATTCGCGCCGGTAGATAC	TGCAATTGCCGGCAATACTA	55.0	45.0	58.9	58.2	1015-1016 bp	1.0	72.0
rbcL-psaI_p2	GCCGGTAGATACCATCGATT	TGCAATTGCCGGCAATACTA	50.0	45.0	57.0	58.2	1007-1008 bp	1.0	71.3
rbcL-psaI_p3	AATTCGCGCCGGTAGATAC	TGCAATTGCCGGCAATACTA	52.6	45.0	57.8	58.2	1014-1015 bp	1.0	70.0
rbcL-psaI_p4	CGCCGGTAGATACCATCGAT	TGCAATTGCCGGCAATACTA	55.0	45.0	59.2	58.2	1008-1009 bp	1.0	70.0
rbcL-psaI_p5	GAATTCGCGCCGGTAGATAC	ATTGCCGGCAATACTAAGCC	55.0	50.0	58.9	58.7	1011-1012 bp	1.0	68.5