Hotspot

ndhC-trnV-UAC

LSC · 50913-51782 · 870 bp

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Pi 0.0116
MarkerSeek score 66.40
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
870
variable_sites
10
pi
0.0116
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CTAACCGGGGCTAAAAGTCC GGTTCGAATCCGTATAGCCC 55.0 55.0 58.0 57.9 991-996 bp 1.0 80.6
ndhC-trnV-UAC_p2 CTAACCGGGGCTAAAAGTCC AGCCCAATGCAGAAAAATGC 55.0 45.0 58.0 57.9 1084-1089 bp 1.0 80.3
ndhC-trnV-UAC_p3 CTAACCGGGGCTAAAAGTCC CGGTTCGAATCCGTATAGCC 55.0 55.0 58.0 58.3 992-997 bp 1.0 78.8
ndhC-trnV-UAC_p4 TAACCGGGGCTAAAAGTCCT GGTTCGAATCCGTATAGCCC 50.0 55.0 58.3 57.9 990-995 bp 1.0 78.2
ndhC-trnV-UAC_p5 TAACCGGGGCTAAAAGTCCT AGCCCAATGCAGAAAAATGC 50.0 45.0 58.3 57.9 1083-1088 bp 1.0 77.9