Hotspot

trnF-GAA-ndhJ

LSC · 48646-49216 · 571 bp

Back to Isachne

Pi 0.0158

MarkerSeek score 65.00

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 571
variable_sites: 9
pi: 0.0158
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GATGCACAAGAGAGGGTCAG	CCATCCACGCCTTAAACGTA	55.0	50.0	58.0	58.0	765-766 bp	1.0	80.7
trnF-GAA-ndhJ_p2	GGATGATGCACAAGAGAGGG	CCATCCACGCCTTAAACGTA	55.0	50.0	58.0	58.0	769-770 bp	1.0	80.5
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	CCATCCACGCCTTAAACGTA	50.0	50.0	58.1	58.0	711-712 bp	1.0	80.5
trnF-GAA-ndhJ_p4	GAGGGTCAGGATAGCTCAGT	CCATCCACGCCTTAAACGTA	55.0	50.0	57.9	58.0	754-755 bp	1.0	80.2
trnF-GAA-ndhJ_p5	GATGCACAAGAGAGGGTCAG	TGAAAGTTGGATAGGCTGGC	55.0	50.0	58.0	57.9	735-736 bp	1.0	79.8