Hotspot

trnT-UGU-trnL-UAA

LSC · 46835-47612 · 778 bp

Back to Isachne

Pi 0.0169

MarkerSeek score 69.70

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 778
variable_sites: 13
pi: 0.0169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	ATTTGAACCGATGACCCTCG	ACAATCAAGTCCGTAGCGTC	50.0	50.0	58.0	58.0	877-879 bp	1.0	81.8
trnT-UGU-trnL-UAA_p2	CGATGACCCTCGCATTACAA	ACAATCAAGTCCGTAGCGTC	50.0	50.0	58.1	58.0	869-871 bp	1.0	81.5
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	ACAATCAAGTCCGTAGCGTC	55.0	50.0	57.9	58.0	847-849 bp	1.0	81.0
trnT-UGU-trnL-UAA_p4	ATTTGAACCGATGACCCTCG	CCGTAGCGTCTACCGATTTC	50.0	55.0	58.0	58.2	867-869 bp	1.0	79.9
trnT-UGU-trnL-UAA_p5	ATTTGAACCGATGACCCTCG	CAATCAAGTCCGTAGCGTCT	50.0	50.0	58.0	57.7	876-878 bp	1.0	79.7