Hotspot

psaA-ycf3

LSC · 42238-42872 · 635 bp

Back to Isachne

Pi 0.0127

MarkerSeek score 66.50

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 635
variable_sites: 8
pi: 0.0127
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GTCTGGCCCATTCCTCAAAA	TATAGCGCTTACTCCGGGAA	50.0	50.0	58.1	58.0	795-796 bp	1.0	80.4
psaA-ycf3_p2	GTTTTTGAGAAATGCCCGGG	TATAGCGCTTACTCCGGGAA	50.0	50.0	57.9	58.0	814-815 bp	1.0	80.3
psaA-ycf3_p3	GTCTGGCCCATTCCTCAAAA	GCGCTTACTCCGGGAAATTA	50.0	50.0	58.1	58.1	791-792 bp	1.0	80.1
psaA-ycf3_p4	GTTTTTGAGAAATGCCCGGG	GCGCTTACTCCGGGAAATTA	50.0	50.0	57.9	58.1	810-811 bp	1.0	80.0
psaA-ycf3_p5	CTGGCCCATTCCTCAAAAGA	TATAGCGCTTACTCCGGGAA	50.0	50.0	57.8	58.0	793-794 bp	1.0	79.2