Hotspot

atpF-atpA

LSC · 35082-35174 · 93 bp

Back to Isachne

Pi 0.0323

MarkerSeek score 71.00

Primer available yes

Label atpF-atpA

Feature Metadata

label_name: atpF-atpA
feature_type: igs
region: LSC
length_bp: 93
variable_sites: 3
pi: 0.0323
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpA_p1	TAACATTGGCATTCTCGGGG	CATCCCCCACTTGAACTACG	50.0	55.0	57.9	58.0	245-246 bp	1.0	80.4
atpF-atpA_p2	CCGTACAAGGAGCTCTAGGA	CATCCCCCACTTGAACTACG	55.0	55.0	57.9	58.0	315-316 bp	1.0	80.1
atpF-atpA_p3	CGATTCGTGCTAACATTGGC	CATCCCCCACTTGAACTACG	50.0	55.0	57.9	58.0	255-256 bp	1.0	80.0
atpF-atpA_p4	AAGCCGTACAAGGAGCTCTA	CATCCCCCACTTGAACTACG	50.0	55.0	58.2	58.0	318-319 bp	1.0	79.3
atpF-atpA_p5	TAACATTGGCATTCTCGGGG	TTGTTCAATACGTTCGCGGA	50.0	45.0	57.9	58.2	190-191 bp	1.0	79.1