Hotspot

atpH-atpF

LSC · 33242-33706 · 465 bp

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Pi 0.00913

MarkerSeek score 65.40

Primer available yes

Label atpH-atpF

Feature Metadata

label_name: atpH-atpF
feature_type: igs
region: LSC
length_bp: 465
variable_sites: 4
pi: 0.00913
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	GCCCTTTTATTTGCGAACCC	CGAATGGCCAGTGAGCTAAA	50.0	50.0	58.0	58.3	517-544 bp	1.0	82.5
atpH-atpF_p2	TTATGGACTGGTTGTGGCAC	CGAATGGCCAGTGAGCTAAA	50.0	50.0	58.1	58.3	539-566 bp	1.0	81.6
atpH-atpF_p3	TTTATGGACTGGTTGTGGCA	CGAATGGCCAGTGAGCTAAA	45.0	50.0	57.3	58.3	540-567 bp	1.0	77.1
atpH-atpF_p4	GCCCTTTTATTTGCGAACCC	TATTAAGCCCGAAACTCCCC	50.0	50.0	58.0	57.0	538-565 bp	1.0	76.3
atpH-atpF_p5	TTATGGACTGGTTGTGGCAC	TATTAAGCCCGAAACTCCCC	50.0	50.0	58.1	57.0	560-587 bp	1.0	75.4