Hotspot

ycf1

SSC · 121375-126735 · 5361 bp

Pi 0.0322

MarkerSeek score 68.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5361
variable_sites: 1191
pi: 0.0322
unique_haplotype_count: 58
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CATACCACTCGGGGAATTGG	TGAACGGACCATATCGAGGA	55.0	50.0	58.0	57.9	969-1086 bp	0.629032	76.4
ycf1_p2	CATACCACTCGGGGAATTGG	TGGAAAAACCGGACATTCGT	55.0	45.0	58.0	58.0	600-606 bp	0.080645	44.3
ycf1_p3	CATACCACTCGGGGAATTGG	GGATCGATGGAAAAACCGGA	55.0	50.0	58.0	58.0	613 bp	0.016129	40.1
ycf1_p4	CATACCACTCGGGGAATTGG	AGGGATCGATGGAAAAACCG	55.0	50.0	58.0	58.0	615 bp	0.016129	40.1
ycf1_p5	CATACCACTCGGGGAATTGG	ACCGGACATTCGTTCAAGAG	55.0	50.0	58.0	57.9	599 bp	0.016129	39.7