Hotspot

psbE-petL

LSC · 63363-64560 · 1198 bp

Pi 0.0286

MarkerSeek score 74.40

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1198
variable_sites: 246
pi: 0.0286
unique_haplotype_count: 51
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	CAATCAACAGAGCTGGGGTT	50.0	50.0	57.9	58.1	1147-1399 bp	1.0	90.9
psbE-petL_p2	ACGAATAACCAACCCGCAAT	CAATCAACAGAGCTGGGGTT	45.0	50.0	58.2	58.1	1156-1408 bp	1.0	90.0
psbE-petL_p3	CGAATAACCAACCCGCAATG	CAATCAACAGAGCTGGGGTT	50.0	50.0	57.8	58.1	1155-1407 bp	1.0	89.8
psbE-petL_p4	ACCAACCCGCAATGAATAGG	CAATCAACAGAGCTGGGGTT	50.0	50.0	58.2	58.1	1149-1401 bp	1.0	89.4
psbE-petL_p5	CCGCAATGAATAGGGAAGGT	CAATCAACAGAGCTGGGGTT	50.0	50.0	57.7	58.1	1143-1395 bp	1.0	88.5