Hotspot

petA-psbJ

LSC · 61748-62593 · 846 bp

Back to Iris

Pi 0.0445
MarkerSeek score 78.40
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
846
variable_sites
225
pi
0.0445
unique_haplotype_count
46
primer_available
yes
alignment_reliability
0.99
markerseek_score
78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 TGAAAAGGTTCAATTGTACGA CATATTCCGGGTTGGGTTCA 33.3 50.0 53.7 57.6 724-922 bp 1.0 52.0
petA-psbJ_p2 TGAAAAGGTTCAATTGTACGA GGTTCATATTCCGGGTTGGG 33.3 55.0 53.7 58.3 728-915 bp 0.967742 50.7
petA-psbJ_p3 AGAAACAGTTTGAAAAGGTTCA GGTTCATATTCCGGGTTGGG 31.8 55.0 54.6 58.3 738-925 bp 0.967742 50.7
petA-psbJ_p4 TGAAAAGGTTCAATTGTACGA GTTCATATTCCGGGTTGGGT 33.3 50.0 53.7 57.6 727-914 bp 0.967742 50.7
petA-psbJ_p5 TGAAAAGGTTCAATTGTACGAA GGTTCATATTCCGGGTTGGG 31.8 55.0 54.5 58.3 728-915 bp 0.967742 50.7