Hotspot

trnL-UAA-trnF-GAA

LSC · 47959-48340 · 382 bp

Back to Iris

Pi 0.0326
MarkerSeek score 72.60
Primer available yes
Label trnL-UAA-trnF-GAA

Feature Metadata

label_name
trnL-UAA-trnF-GAA
feature_type
igs
region
LSC
length_bp
382
variable_sites
88
pi
0.0326
unique_haplotype_count
43
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAA-trnF-GAA_p1 AATCGTGAGGGTTCAAGTCC TTTGAACTGGTGACACGAGG 50.0 50.0 57.5 58.1 423-493 bp 1.0 87.8
trnL-UAA-trnF-GAA_p2 AATCGTGAGGGTTCAAGTCC GAACTGGTGACACGAGGATT 50.0 50.0 57.5 57.5 420-490 bp 1.0 84.5
trnL-UAA-trnF-GAA_p3 TTCAAGTCCCTCTATCCCCA TTTGAACTGGTGACACGAGG 50.0 50.0 57.0 58.1 412-482 bp 1.0 83.9
trnL-UAA-trnF-GAA_p4 AATCGTGAGGGTTCAAGTCC TCCTCTGCTCTACCAACTGA 50.0 50.0 57.5 57.4 395-465 bp 1.0 83.4
trnL-UAA-trnF-GAA_p5 AATCGTGAGGGTTCAAGTCC TACCAACTGAGCTATCCCGA 50.0 50.0 57.5 57.6 385-455 bp 0.919355 81.4