Hotspot

rpoB-trnC-GCA

LSC · 26175-27377 · 1203 bp

Back to Iris

Pi 0.0389
MarkerSeek score 76.60
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1203
variable_sites
304
pi
0.0389
unique_haplotype_count
58
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 CCAAATTAATCCCGCGGGTA CGGGGCAAGAATTCGTCTAA 50.0 50.0 58.0 58.0 1237-1637 bp 0.870968 86.5
rpoB-trnC-GCA_p2 TCGAGGGCTCTACCAATTGA CGGGGCAAGAATTCGTCTAA 50.0 50.0 58.1 58.0 1215-1563 bp 0.758065 81.1
rpoB-trnC-GCA_p3 CCAAATTAATCCCGCGGGTA CCGAACTCCGACTTTCGTAG 50.0 55.0 58.0 58.1 1334-1902 bp 0.741935 80.6
rpoB-trnC-GCA_p4 CCAAATTAATCCCGCGGGTA ACCAAGATTTCCGAACTCCG 50.0 50.0 58.0 57.9 1344-1912 bp 0.435484 68.4
rpoB-trnC-GCA_p5 CCAAATTAATCCCGCGGGTA GGATTTGAACCGGGGATGAA 50.0 50.0 58.0 57.9 1524-1536 bp 0.112903 45.5