Hotspot

trnS-GCU-trnG-UCC

LSC · 7978-8866 · 889 bp

Back to Iris

Pi 0.0372
MarkerSeek score 75.60
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
889
variable_sites
239
pi
0.0372
unique_haplotype_count
48
primer_available
yes
alignment_reliability
0.96
markerseek_score
75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 AATCTGCCGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 58.3 55.5 914-957 bp 1.0 61.6
trnS-GCU-trnG-UCC_p2 CAATCTGCCGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 58.3 55.5 915-958 bp 1.0 61.6
trnS-GCU-trnG-UCC_p3 CGGAGAGAGAGGGATTCGAA ACGAATCACACTTTTACCACT 55.0 38.1 58.3 55.5 968-1011 bp 1.0 61.2
trnS-GCU-trnG-UCC_p4 AATCTGCCGCTTTAGTCCAC AGAACGAATCACACTTTTACCAC 50.0 39.1 58.3 57.3 917-960 bp 1.0 60.6
trnS-GCU-trnG-UCC_p5 AATCTGCCGCTTTAGTCCAC GAACGAATCACACTTTTACCACT 50.0 39.1 58.3 57.3 916-959 bp 1.0 60.6