Hotspot

psbK-psbI

LSC · 7267-7652 · 386 bp

Back to Iris

Pi 0.0375
MarkerSeek score 73.10
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
386
variable_sites
104
pi
0.0375
unique_haplotype_count
55
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 GTTTGGCAAGCTGCTGTAAG ACAAAGAGTTTGAGAGTAAGCA 50.0 36.4 57.9 55.6 120-460 bp 1.0 56.4
psbK-psbI_p2 TGGCAAGCTGCTGTAAGTTT ACAAAGAGTTTGAGAGTAAGCA 45.0 36.4 58.3 55.6 117-457 bp 1.0 54.4
psbK-psbI_p3 TTGGCAAGCTGCTGTAAGTT ACAAAGAGTTTGAGAGTAAGCA 45.0 36.4 58.3 55.6 118-458 bp 1.0 54.4
psbK-psbI_p4 TTTGGCAAGCTGCTGTAAGT ACAAAGAGTTTGAGAGTAAGCA 45.0 36.4 58.3 55.6 119-459 bp 1.0 54.4
psbK-psbI_p5 GTTTGGCAAGCTGCTGTAAG AACAAAGAGTTTGAGAGTAAGCA 50.0 34.8 57.9 56.3 121-461 bp 1.0 54.2