Hotspot

rps15-trnN-GUU

IRa · 132702-139439 · 6738 bp

Back to Ipomoea

Pi 0.00444

MarkerSeek score 60.80

Primer available yes

Label rps15-trnN-GUU

Feature Metadata

label_name: rps15-trnN-GUU
feature_type: igs
region: IRa
length_bp: 6738
variable_sites: 116
pi: 0.00444
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-trnN-GUU_p1	TAAAATCGGGTTGGTGTGCA	ACAGGTGAGCAAAATCGGTT	45.0	45.0	58.0	58.0	667-892 bp	1.0	88.3
rps15-trnN-GUU_p2	TAAAATCGGGTTGGTGTGCA	GCTTCCGCCTTCCATCATAA	45.0	50.0	58.0	58.0	716-941 bp	1.0	88.0
rps15-trnN-GUU_p3	GCATCAAAACAAGCCCCTTC	ACAGGTGAGCAAAATCGGTT	50.0	45.0	57.9	58.0	643-868 bp	1.0	87.9
rps15-trnN-GUU_p4	GTATCCCAGGCTTCCCCTAT	ACAGGTGAGCAAAATCGGTT	55.0	45.0	58.0	58.0	980-1205 bp	1.0	87.2
rps15-trnN-GUU_p5	GTATCCCAGGCTTCCCCTAT	GCTTCCGCCTTCCATCATAA	55.0	50.0	58.0	58.0	1029-1254 bp	1.0	87.0