Hotspot

rpl32-trnL-UAG

SSC · 122117-123031 · 915 bp

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Pi 0.0284
MarkerSeek score 81.90
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
915
variable_sites
103
pi
0.0284
unique_haplotype_count
18
primer_available
yes
alignment_reliability
0.95
markerseek_score
81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGGAAAATCCCTTTCGACCG TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.9 57.9 986-1038 bp 0.95 87.8
rpl32-trnL-UAG_p2 CGACCGGGAATTTGAAAAGC TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.0 57.9 1013 bp 0.05 40.8
rpl32-trnL-UAG_p3 CGACCGGGAATTTGAAAAGC AGCAGCGTGTCTACCAATTT 50.0 45.0 58.0 57.8 1006 bp 0.05 40.3
rpl32-trnL-UAG_p4 CGACCGGGAATTTGAAAAGC CCTAAGAGCAGCGTGTCTAC 50.0 55.0 58.0 57.8 1012 bp 0.05 40.2
rpl32-trnL-UAG_p5 CGACCGGGAATTTGAAAAGC CTAAGAGCAGCGTGTCTACC 50.0 55.0 58.0 57.8 1011 bp 0.05 40.2