Hotspot

ndhF-rpl32

SSC · 121289-121942 · 654 bp

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Pi 0.0124
MarkerSeek score 65.00
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
654
variable_sites
46
pi
0.0124
unique_haplotype_count
15
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 ACAAAAGGTATGATCCACGCA TGCTGTCCAAAAGCTCTTTCT 42.9 42.9 57.9 58.1 779-806 bp 1.0 69.4
ndhF-rpl32_p2 ACAAAAGGTATGATCCACGCA TGCTGTCCAAAAGCTCTTTC 42.9 45.0 57.9 56.5 779-806 bp 1.0 66.3
ndhF-rpl32_p3 ACAAAAGGTATGATCCACGCA GCTGTCCAAAAGCTCTTTCT 42.9 45.0 57.9 56.2 778-805 bp 1.0 63.9
ndhF-rpl32_p4 CAAAAGGTATGATCCACGCA TGCTGTCCAAAAGCTCTTTCT 45.0 42.9 56.1 58.1 778-805 bp 1.0 63.0
ndhF-rpl32_p5 ACAAAAGGTATGATCCACGC TGCTGTCCAAAAGCTCTTTCT 45.0 42.9 56.1 58.1 779-806 bp 1.0 62.9