Hotspot

ndhH-ndhF

SSC · 118078-119155 · 1078 bp

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Pi 0.014

MarkerSeek score 62.00

Primer available yes

Label ndhH-ndhF

Feature Metadata

label_name: ndhH-ndhF
feature_type: igs
region: SSC
length_bp: 1078
variable_sites: 67
pi: 0.014
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH-ndhF_p1	ATGGGGGAAGTTGATCGTTG	AGGATATGCGAGAATTCCTT	50.0	40.0	57.9	53.9	1154-1199 bp	1.0	54.6
ndhH-ndhF_p2	ATGGGGGAAGTTGATCGTTG	AGGATATGCGAGAATTCCTTT	50.0	38.1	57.9	54.8	1154-1199 bp	1.0	53.7
ndhH-ndhF_p3	ATGGGGGAAGTTGATCGTTG	AGGATATGCGAGAATTCCTTTT	50.0	36.4	57.9	55.6	1154-1199 bp	1.0	52.1
ndhH-ndhF_p4	GTCCACCGGGTTTGATCAAT	AGGATATGCGAGAATTCCTT	50.0	40.0	58.2	53.9	1252-1297 bp	0.95	51.7
ndhH-ndhF_p5	GTCCACCGGGTTTGATCAAT	AGGATATGCGAGAATTCCTTT	50.0	38.1	58.2	54.8	1252-1297 bp	0.95	50.7