Hotspot

trnN-GUU-rps15

IRb · 109763-116500 · 6738 bp

Back to Ipomoea

Pi 0.00423
MarkerSeek score 62.20
Primer available yes
Label trnN-GUU-rps15

Feature Metadata

label_name
trnN-GUU-rps15
feature_type
igs
region
IRb
length_bp
6738
variable_sites
113
pi
0.00423
unique_haplotype_count
20
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-rps15_p1 ACAGGTGAGCAAAATCGGTT TAAAATCGGGTTGGTGTGCA 45.0 45.0 58.0 58.0 667-892 bp 1.0 87.9
trnN-GUU-rps15_p2 GCTTCCGCCTTCCATCATAA TAAAATCGGGTTGGTGTGCA 50.0 45.0 58.0 58.0 716-941 bp 1.0 87.6
trnN-GUU-rps15_p3 ACAGGTGAGCAAAATCGGTT GCATCAAAACAAGCCCCTTC 45.0 50.0 58.0 57.9 643-868 bp 1.0 87.5
trnN-GUU-rps15_p4 ACAGGTGAGCAAAATCGGTT GTATCCCAGGCTTCCCCTAT 45.0 55.0 58.0 58.0 980-1205 bp 1.0 86.9
trnN-GUU-rps15_p5 GCTTCCGCCTTCCATCATAA GTATCCCAGGCTTCCCCTAT 50.0 55.0 58.0 58.0 1029-1254 bp 1.0 86.7