Hotspot

petA-psbJ

LSC · 65049-65920 · 872 bp

Back to Ipomoea

Pi 0.00574

MarkerSeek score 61.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 872
variable_sites: 27
pi: 0.00574
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCGTCTGTTATTTTGGCAC	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.0	57.6	976-1014 bp	1.0	80.8
petA-psbJ_p2	CGAGAAAGTTCAATTGGCCG	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.8	57.6	925-963 bp	1.0	79.2
petA-psbJ_p3	TGGCGTCTGTTATTTTGGCA	ATTCCGGATTGGGTTCATCC	45.0	50.0	58.4	57.6	977-1015 bp	1.0	77.9
petA-psbJ_p4	ACGTGTCCAAGGACTTTTGT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.6	57.6	1003-1041 bp	1.0	77.6
petA-psbJ_p5	GCGTCTGTTATTTTGGCACA	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.3	57.6	975-1013 bp	1.0	75.4