Hotspot

trnT-UGU-trnL-UAA

LSC · 48507-49381 · 875 bp

Back to Ipomoea

Pi 0.00822

MarkerSeek score 61.20

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 875
variable_sites: 39
pi: 0.00822
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	AAAGCCAGCTATCGGAATCG	CCGTAGCGTCTACCGATTTC	50.0	55.0	58.1	58.2	969-1032 bp	1.0	84.1
trnT-UGU-trnL-UAA_p2	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	956-1019 bp	1.0	83.3
trnT-UGU-trnL-UAA_p3	AAAGCCAGCTATCGGAATCG	CGTCTACCGATTTCGCCATA	50.0	50.0	58.1	57.6	963-1026 bp	1.0	82.8
trnT-UGU-trnL-UAA_p4	AAATGCGATGCTCTAACCCC	CCGTAGCGTCTACCGATTTC	50.0	55.0	58.3	58.2	928-991 bp	1.0	82.7
trnT-UGU-trnL-UAA_p5	GGAATCGAACCGATGACCAT	CGTCTACCGATTTCGCCATA	50.0	50.0	57.8	57.6	950-1013 bp	1.0	82.0