Hotspot

trnM-CAU-psbD

LSC · 32976-34317 · 1342 bp

Back to Ipomoea

Pi 0.00471

MarkerSeek score 60.20

Primer available yes

Label trnM-CAU-psbD

Feature Metadata

label_name: trnM-CAU-psbD
feature_type: igs
region: LSC
length_bp: 1342
variable_sites: 41
pi: 0.00471
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-psbD_p1	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	55.0	50.0	58.1	58.1	1449-1509 bp	1.0	84.5
trnM-CAU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.1	58.1	1452-1512 bp	1.0	84.4
trnM-CAU-psbD_p3	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1439-1499 bp	1.0	83.5
trnM-CAU-psbD_p4	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1442-1502 bp	1.0	83.3
trnM-CAU-psbD_p5	GGCGTAAGTCATCGGTTCAA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.3	58.1	1426-1486 bp	1.0	82.9