Hotspot

trnE-UUC-trnT-GGU

LSC · 32155-32910 · 756 bp

Back to Ipomoea

Pi 0.00945
MarkerSeek score 62.90
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
756
variable_sites
36
pi
0.00945
unique_haplotype_count
17
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 CATAGTACCCTACCCCCAGG GAACCGATGACTTACGCCTT 60.0 50.0 58.0 58.0 876-897 bp 1.0 86.5
trnE-UUC-trnT-GGU_p2 CATAGTACCCTACCCCCAGG CCATGGCGTTACTCTACCAC 60.0 55.0 58.0 58.1 855-876 bp 1.0 86.2
trnE-UUC-trnT-GGU_p3 CATAGTACCCTACCCCCAGG TGGCGTTACTCTACCACTGA 60.0 50.0 58.0 58.1 852-873 bp 1.0 86.0
trnE-UUC-trnT-GGU_p4 CATAGTACCCTACCCCCAGG CCGATGACTTACGCCTTACC 60.0 55.0 58.0 58.2 873-894 bp 1.0 85.4
trnE-UUC-trnT-GGU_p5 GCTGCCTCCTTGAAAGAGAG GAACCGATGACTTACGCCTT 55.0 50.0 58.3 58.0 841-862 bp 1.0 84.9