Hotspot

psbM-trnD-GUC

LSC · 31088-31762 · 675 bp

Back to Ipomoea

Pi 0.0134

MarkerSeek score 62.60

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 675
variable_sites: 31
pi: 0.0134
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AAGGAGAAAGGCGGTAGGAA	TTCAATTGGTCAGAGCACCG	50.0	50.0	58.3	58.5	782-808 bp	1.0	80.0
psbM-trnD-GUC_p2	TAAGGAGAAAGGCGGTAGGA	TTCAATTGGTCAGAGCACCG	50.0	50.0	57.5	58.5	783-809 bp	1.0	78.4
psbM-trnD-GUC_p3	AAGGAGAAAGGCGGTAGGAA	GTTCAATTGGTCAGAGCACC	50.0	50.0	58.3	57.0	783-809 bp	1.0	75.8
psbM-trnD-GUC_p4	ATAAGGAGAAAGGCGGTAGGA	TTCAATTGGTCAGAGCACCG	47.6	50.0	58.0	58.5	784-810 bp	1.0	74.4
psbM-trnD-GUC_p5	TAAGGAGAAAGGCGGTAGGA	GTTCAATTGGTCAGAGCACC	50.0	50.0	57.5	57.0	784-810 bp	1.0	74.3