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petN-psbM

LSC · 29762-30982 · 1221 bp

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Pi 0.0155

MarkerSeek score 57.10

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1221
variable_sites: 85
pi: 0.0155
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	CGGAAACCCCAAGTCAAGAA	GTGGAAGGACGCTTTGATGA	50.0	50.0	58.0	58.2	1919-2195 bp	1.0	86.8
petN-psbM_p2	TTCGGAAACCCCAAGTCAAG	GTGGAAGGACGCTTTGATGA	50.0	50.0	58.0	58.2	1921-2197 bp	1.0	86.8
petN-psbM_p3	CGGAAACCCCAAGTCAAGAA	TAATCCAAGGCAAGGTTGGG	50.0	50.0	58.0	57.8	1808-2080 bp	0.95	84.4
petN-psbM_p4	CGGAAACCCCAAGTCAAGAA	AGTGGAAGGACGCTTTGATG	50.0	50.0	58.0	58.2	1920-2196 bp	0.75	76.2
petN-psbM_p5	TTCGGAAACCCCAAGTCAAG	AGTGGAAGGACGCTTTGATG	50.0	50.0	58.0	58.2	1922-2198 bp	0.75	76.2