Hotspot

trnL-CAA-ycf15

IRa · 147182-147529 · 348 bp

Back to Iochroma

Pi 0.00623

MarkerSeek score 54.00

Primer available yes

Label trnL-CAA-ycf15

Feature Metadata

label_name: trnL-CAA-ycf15
feature_type: igs
region: IRa
length_bp: 348
variable_sites: 5
pi: 0.00623
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ycf15_p1	AAATGGTAGACACGCGAGAC	AGGAGAAGGAAGGCTGGAAT	50.0	50.0	58.0	58.0	389-486 bp	1.0	84.6
trnL-CAA-ycf15_p2	TGAAATGGTAGACACGCGAG	AGGAGAAGGAAGGCTGGAAT	50.0	50.0	58.0	58.0	391-488 bp	1.0	84.6
trnL-CAA-ycf15_p3	AAATGGTAGACACGCGAGAC	GGAGAAGGAAGGCTGGAATG	50.0	55.0	58.0	58.0	388-485 bp	1.0	84.4
trnL-CAA-ycf15_p4	TGAAATGGTAGACACGCGAG	GGAGAAGGAAGGCTGGAATG	50.0	55.0	58.0	58.0	390-487 bp	1.0	84.4
trnL-CAA-ycf15_p5	GCCTTGGTGGTGAAATGGTA	AGGAGAAGGAAGGCTGGAAT	50.0	50.0	58.1	58.0	401-498 bp	1.0	84.0