Hotspot

rpl32-trnL-UAG

SSC · 116178-117002 · 825 bp

Back to Iochroma

Pi 0.00333
MarkerSeek score 49.70
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
825
variable_sites
6
pi
0.00333
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGGAAGGGATATTGGGTAGCA TCCTAAGAGCAGCGTGTCTA 47.6 50.0 57.9 57.9 952-970 bp 1.0 72.5
rpl32-trnL-UAG_p2 AGGAAGGGATATTGGGTAGCA TCGAACCGAGATGCATTAGC 47.6 50.0 57.9 58.1 978-996 bp 1.0 72.2
rpl32-trnL-UAG_p3 AGGAAGGGATATTGGGTAGCA AGCAGCGTGTCTACCAATTT 47.6 45.0 57.9 57.8 945-963 bp 1.0 72.0
rpl32-trnL-UAG_p4 AGGAAGGGATATTGGGTAGCA CCTAAGAGCAGCGTGTCTAC 47.6 55.0 57.9 57.8 951-969 bp 1.0 71.9
rpl32-trnL-UAG_p5 AGGAAGGGATATTGGGTAGCA CTAAGAGCAGCGTGTCTACC 47.6 55.0 57.9 57.8 950-968 bp 1.0 71.9