Hotspot

ycf15-trnL-CAA

IRb · 96761-97108 · 348 bp

Back to Iochroma

Pi 0.00582
MarkerSeek score 51.10
Primer available yes
Label ycf15-trnL-CAA

Feature Metadata

label_name
ycf15-trnL-CAA
feature_type
igs
region
IRb
length_bp
348
variable_sites
4
pi
0.00582
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf15-trnL-CAA_p1 AGGAGAAGGAAGGCTGGAAT AAATGGTAGACACGCGAGAC 50.0 50.0 58.0 58.0 389-474 bp 1.0 84.5
ycf15-trnL-CAA_p2 AGGAGAAGGAAGGCTGGAAT TGAAATGGTAGACACGCGAG 50.0 50.0 58.0 58.0 391-476 bp 1.0 84.4
ycf15-trnL-CAA_p3 GGAGAAGGAAGGCTGGAATG AAATGGTAGACACGCGAGAC 55.0 50.0 58.0 58.0 388-473 bp 1.0 84.2
ycf15-trnL-CAA_p4 GGAGAAGGAAGGCTGGAATG TGAAATGGTAGACACGCGAG 55.0 50.0 58.0 58.0 390-475 bp 1.0 84.2
ycf15-trnL-CAA_p5 AGGAGAAGGAAGGCTGGAAT GCCTTGGTGGTGAAATGGTA 50.0 50.0 58.0 58.1 401-486 bp 1.0 83.8