Hotspot

clpP-psbT

LSC · 75189-77368 · 2180 bp

Back to Iochroma

Pi 0.000901

MarkerSeek score 48.70

Primer available yes

Label clpP-psbT

Feature Metadata

label_name: clpP-psbT
feature_type: igs
region: LSC
length_bp: 2180
variable_sites: 5
pi: 0.000901
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbT_p1	GGGGAAATACCATGTGACCC	GAACTTTAGGCGGTTCTCGA	55.0	50.0	57.9	57.6	2380 bp	1.0	76.8
clpP-psbT_p2	GGGGAAATACCATGTGACCC	TGGAACTTTAGGCGGTTCTC	55.0	50.0	57.9	57.5	2382 bp	1.0	75.9
clpP-psbT_p3	CGGGGAAATACCATGTGACC	GAACTTTAGGCGGTTCTCGA	55.0	50.0	58.3	57.6	2381 bp	1.0	74.7
clpP-psbT_p4	GGGGAAATACCATGTGACCC	GGAACTTTAGGCGGTTCTCG	55.0	55.0	57.9	58.6	2381 bp	1.0	74.5
clpP-psbT_p5	GGGGAAATACCATGTGACCC	AGTTGGAACTTTAGGCGGTT	55.0	45.0	57.9	57.3	2385 bp	1.0	74.4