Hotspot

petA-psbJ

LSC · 65931-67003 · 1073 bp

Back to Iochroma

Pi 0.00283

MarkerSeek score 40.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1073
variable_sites: 8
pi: 0.00283
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGGATTTGGTCAGGGAGATG	AACTGGCCGTTTTGATCCTT	55.0	45.0	58.0	58.0	1434-1800 bp	1.0	84.0
petA-psbJ_p2	CGGATTTGGTCAGGGAGATG	ACTGGCCGTTTTGATCCTTT	55.0	45.0	58.0	58.0	1433-1799 bp	1.0	84.0
petA-psbJ_p3	CGGATTTGGTCAGGGAGATG	TGCGGGTTGGTTATTTGTCA	55.0	45.0	58.0	58.0	1536-1902 bp	1.0	83.9
petA-psbJ_p4	GCTCCTCCCGATCGTATTTC	AACTGGCCGTTTTGATCCTT	55.0	45.0	57.9	58.0	1886-2252 bp	1.0	83.8
petA-psbJ_p5	GCTCCTCCCGATCGTATTTC	ACTGGCCGTTTTGATCCTTT	55.0	45.0	57.9	58.0	1885-2251 bp	1.0	83.8