Hotspot

petN-psbM

LSC · 29657-30758 · 1102 bp

Back to Iochroma

Pi 0.00243

MarkerSeek score 50.10

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1102
variable_sites: 7
pi: 0.00243
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	AGTTCCTACCGCTTTTCTACT	45.0	42.9	58.0	56.4	1236 bp	1.0	59.5
petN-psbM_p2	AGTAAGTCTTGCTTGGGCTG	AGTTCCTACCGCTTTTCTACT	50.0	42.9	57.8	56.4	1242 bp	1.0	58.0
petN-psbM_p3	GGGGAAGGAGTGGACTCTAG	AGTTCCTACCGCTTTTCTACT	60.0	42.9	58.2	56.4	1180 bp	1.0	57.9
petN-psbM_p4	TCTTGCTTGGGCTGCTTTAA	AGTTCCTACCGCTTTTCTACTT	45.0	40.9	58.0	57.1	1236 bp	1.0	57.3
petN-psbM_p5	TCTTGCTTGGGCTGCTTTAA	TCTAGTTCCTACCGCTTTTCT	45.0	42.9	58.0	56.1	1239 bp	1.0	57.3