Hotspot

ycf1

SSC · 121683-126776 · 5094 bp

Pi 0.0119

MarkerSeek score 46.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5094
variable_sites: 118
pi: 0.0119
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGGGGCGATTCCACATTATT	ATCCTTTTGGTCCTCCTCGA	50.0	50.0	58.0	58.0	424 bp	1.0	83.0
ycf1_p2	GGGGGCGATTCCACATTATT	CGGTTTTGGGAATGGAAACG	50.0	50.0	58.0	57.9	448 bp	1.0	82.9
ycf1_p3	GGGGGCGATTCCACATTATT	AACAACCAGGAAGACGCAAT	50.0	45.0	58.0	58.0	833 bp	1.0	82.1
ycf1_p4	CAAAGTTTTGGGGGCGATTC	AACAACCAGGAAGACGCAAT	50.0	45.0	57.9	58.0	842 bp	1.0	81.5
ycf1_p5	AGTTGTAGAGCGATTCTGCG	AACAACCAGGAAGACGCAAT	50.0	45.0	58.1	58.0	953 bp	1.0	81.4