Hotspot

rbcL

LSC · 53693-55123 · 1431 bp

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Pi 0.0108
MarkerSeek score 54.50
Primer available yes
Label rbcL

Feature Metadata

label_name
rbcL
feature_type
gene
region
LSC
length_bp
1431
variable_sites
30
pi
0.0108
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL_p1 TCATGAGTTGTAGGGAGGGA TCCAAAGTATCCATTGTCTG 50.0 40.0 57.1 52.6 1478 bp 1.0 41.6
rbcL_p2 TCATGAGTTGTAGGGAGGGA TCCTTATCCAAAGTATCCATTGTCT 50.0 36.0 57.1 57.5 1484 bp 1.0 41.6
rbcL_p3 TCATGAGTTGTAGGGAGGGA CCTTATCCAAAGTATCCATTGTCTG 50.0 40.0 57.1 57.5 1483 bp 1.0 41.6
rbcL_p4 TTCATGAGTTGTAGGGAGGGA TCCAAAGTATCCATTGTCTG 47.6 40.0 57.8 52.6 1479 bp 1.0 41.6
rbcL_p5 TTCATGAGTTGTAGGGAGGGA TCCTTATCCAAAGTATCCATTGTCT 47.6 36.0 57.8 57.5 1485 bp 1.0 41.6