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ndhF-rpl32

SSC · 110038-111051 · 1014 bp

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Pi 0.0191
MarkerSeek score 53.30
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1014
variable_sites
36
pi
0.0191
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GTTGCCGTCGGAAAAAGAAG TCCAAATATTTTTACGAATACGCT 50.0 29.2 58.0 55.3 1173-1241 bp 1.0 42.8
ndhF-rpl32_p2 TGCCGTCGGAAAAAGAAGAA TCCAAATATTTTTACGAATACGCT 45.0 29.2 58.0 55.3 1171-1239 bp 1.0 42.8
ndhF-rpl32_p3 TTGCCGTCGGAAAAAGAAGA TCCAAATATTTTTACGAATACGCT 45.0 29.2 58.0 55.3 1172-1240 bp 1.0 42.8
ndhF-rpl32_p4 CTTTTTGTTGCCGTCGGAAA TCCAAATATTTTTACGAATACGCT 45.0 29.2 57.8 55.3 1179-1247 bp 1.0 42.8
ndhF-rpl32_p5 TTTGTTGCCGTCGGAAAAAG TCCAAATATTTTTACGAATACGCT 45.0 29.2 57.8 55.3 1176-1244 bp 1.0 42.8