Hotspot

clpP-psbB

LSC · 70520-70969 · 450 bp

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Pi 0.0144
MarkerSeek score 57.90
Primer available yes
Label clpP-psbB

Feature Metadata

label_name
clpP-psbB
feature_type
igs
region
LSC
length_bp
450
variable_sites
12
pi
0.0144
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP-psbB_p1 TCCTCTCCAGGACTTCGAAA CGATACCAAGGCAAACCCAT 50.0 50.0 57.7 58.2 516-517 bp 1.0 77.7
clpP-psbB_p2 TCCTCTCCAGGACTTCGAAA AGCAACCAAAGCTGTATGCA 50.0 45.0 57.7 58.4 589-590 bp 1.0 76.3
clpP-psbB_p3 TCCTCTCCAGGACTTCGAAA ACACGATACCAAGGCAAACC 50.0 50.0 57.7 58.5 519-520 bp 1.0 75.8
clpP-psbB_p4 GATGCATCTTCCTCTCCAGG CGATACCAAGGCAAACCCAT 55.0 50.0 57.5 58.2 525-526 bp 1.0 75.7
clpP-psbB_p5 TCCTCTCCAGGACTTCGAAA TGAACACGATACCAAGGCAA 50.0 45.0 57.7 57.4 522-523 bp 1.0 75.1