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rps12-clpP

LSC · 68347-68507 · 161 bp

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Pi 0.0135
MarkerSeek score 53.70
Primer available yes
Label rps12-clpP

Feature Metadata

label_name
rps12-clpP
feature_type
igs
region
LSC
length_bp
161
variable_sites
4
pi
0.0135
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.91
markerseek_score
53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-clpP_p1 GTACATGTTCCTCGACGCTG GCAACAGAGGCTCAAGCTTA 55.0 50.0 58.7 58.2 301-316 bp 1.0 77.6
rps12-clpP_p2 AGTACATGTTCCTCGACGCT GCAACAGAGGCTCAAGCTTA 50.0 50.0 58.8 58.2 302-317 bp 1.0 76.8
rps12-clpP_p3 TACATGTTCCTCGACGCTGA GCAACAGAGGCTCAAGCTTA 50.0 50.0 58.8 58.2 300-315 bp 1.0 76.8
rps12-clpP_p4 TAGTACATGTTCCTCGACGC GCAACAGAGGCTCAAGCTTA 50.0 50.0 57.2 58.2 303-318 bp 1.0 76.7
rps12-clpP_p5 GTACATGTTCCTCGACGCTG GTACAAAGAACGGGCAAACC 55.0 50.0 58.7 57.6 367-382 bp 1.0 74.9