Hotspot

petA-psbJ

LSC · 61655-62423 · 769 bp

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Pi 0.0127
MarkerSeek score 57.10
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
769
variable_sites
19
pi
0.0127
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 ACGTGTACAAGGCCTTTTGT ATTCCGGATTGGGTTCATCC 45.0 50.0 58.0 57.6 902-907 bp 1.0 79.3
petA-psbJ_p2 GGCCTTTTGTTCTTCTTCGC ATTCCGGATTGGGTTCATCC 50.0 50.0 57.9 57.6 892-897 bp 1.0 79.3
petA-psbJ_p3 ACGTGTACAAGGCCTTTTGT TGTGATCGGTTTAGTAGGCG 45.0 50.0 58.0 57.4 940-945 bp 1.0 77.5
petA-psbJ_p4 ACGTGTACAAGGCCTTTTGT GTGATCGGTTTAGTAGGCGT 45.0 50.0 58.0 57.4 939-944 bp 1.0 77.5
petA-psbJ_p5 GGCCTTTTGTTCTTCTTCGC TGTGATCGGTTTAGTAGGCG 50.0 50.0 57.9 57.4 930-935 bp 1.0 77.5