Hotspot

ycf4-cemA

LSC · 58899-59771 · 873 bp

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Pi 0.0169
MarkerSeek score 61.10
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
873
variable_sites
29
pi
0.0169
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 GAGCCATTCCATTGACTCGT AGAGAGAAATCCACCAGGGT 50.0 50.0 58.0 57.6 1043-1056 bp 1.0 80.2
ycf4-cemA_p2 TTGGCCTATTTCTTGCGTGT AGAGAGAAATCCACCAGGGT 45.0 50.0 58.1 57.6 975-988 bp 1.0 79.9
ycf4-cemA_p3 CTCCACGGGAAATGGAACAA AGAGAGAAATCCACCAGGGT 50.0 50.0 58.1 57.6 1007-1020 bp 1.0 79.8
ycf4-cemA_p4 AAAAAGCTGCTGAATTGGCC AGAGAGAAATCCACCAGGGT 45.0 50.0 57.8 57.6 989-1002 bp 1.0 79.3
ycf4-cemA_p5 AAAGCTGCTGAATTGGCCTA AGAGAGAAATCCACCAGGGT 45.0 50.0 57.8 57.6 987-1000 bp 1.0 78.9