Hotspot

trnF-GAA-ndhJ

LSC · 47296-47914 · 619 bp

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Pi 0.0105
MarkerSeek score 56.00
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
619
variable_sites
11
pi
0.0105
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.87
markerseek_score
56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 CCTCGTGTCACCAGTTCAAA TGGATAGGATGGCCTTTACG 50.0 50.0 58.1 56.8 672-722 bp 1.0 75.1
trnF-GAA-ndhJ_p2 CCTCGTGTCACCAGTTCAAA GGATAGGATGGCCTTTACGT 50.0 50.0 58.1 56.8 671-721 bp 1.0 75.1
trnF-GAA-ndhJ_p3 CCTCGTGTCACCAGTTCAAA TGAAAGTTGGATAGGATGGCC 50.0 47.6 58.1 57.7 679-729 bp 1.0 74.5
trnF-GAA-ndhJ_p4 CCTCGTGTCACCAGTTCAAA TTGGATAGGATGGCCTTTACG 50.0 47.6 58.1 57.5 673-723 bp 1.0 73.0
trnF-GAA-ndhJ_p5 CCTCGTGTCACCAGTTCAAA TGGATAGGATGGCCTTTACGT 50.0 47.6 58.1 58.6 672-722 bp 1.0 72.1