Hotspot

psaA-ycf3

LSC · 41034-41749 · 716 bp

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Pi 0.0149
MarkerSeek score 49.50
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
716
variable_sites
18
pi
0.0149
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.93
markerseek_score
49.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 GCATCAGCATGTAGGTTCCA TTCTGAAATTGCGGAGGCTT 50.0 45.0 58.0 58.1 945-1006 bp 1.0 84.7
psaA-ycf3_p2 AACGCTGCAAAGATCAATGC TTCTGAAATTGCGGAGGCTT 45.0 45.0 58.0 58.1 1293-1354 bp 1.0 83.8
psaA-ycf3_p3 GTTATTCCAGATGCTCGCCA TTCTGAAATTGCGGAGGCTT 50.0 45.0 58.0 58.1 1239-1300 bp 1.0 83.7
psaA-ycf3_p4 AACGCTGCAAAGATCAATGC ATTCTGAAATTGCGGAGGCT 45.0 45.0 58.0 57.9 1294-1355 bp 1.0 83.4
psaA-ycf3_p5 CCCCGCTAAATGGTGATTCA TTCTGAAATTGCGGAGGCTT 50.0 45.0 57.9 58.1 1390-1451 bp 1.0 83.2