Hotspot

atpI-atpH

LSC · 24846-26024 · 1179 bp

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Pi 0.00923
MarkerSeek score 55.10
Primer available yes
Label atpI-atpH

Feature Metadata

label_name
atpI-atpH
feature_type
igs
region
LSC
length_bp
1179
variable_sites
19
pi
0.00923
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.91
markerseek_score
55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpI-atpH_p1 CCCGTCATGTTCCTTGGATT ATAACGGAAGCGGCAGAAAT 50.0 45.0 57.9 58.0 1209-1313 bp 1.0 83.9
atpI-atpH_p2 CCCGTCATGTTCCTTGGATT TACCTTGACCAACTCCAGGT 50.0 50.0 57.9 57.9 1265-1369 bp 1.0 83.2
atpI-atpH_p3 CCCGTCATGTTCCTTGGATT AGCCAATCCAGCAGCAATAA 50.0 45.0 57.9 57.9 1225-1329 bp 1.0 83.0
atpI-atpH_p4 CCCGTCATGTTCCTTGGATT AATAACGGAAGCGGCAGAAA 50.0 45.0 57.9 58.2 1210-1314 bp 1.0 82.7
atpI-atpH_p5 CCCGTCATGTTCCTTGGATT GCAGTACCTTGACCAACTCC 50.0 55.0 57.9 58.2 1269-1373 bp 1.0 82.6