Hotspot

psbA-trnK-UUU

LSC · 1542-1761 · 220 bp

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Pi 0.0247
MarkerSeek score 63.50
Primer available yes
Label psbA-trnK-UUU

Feature Metadata

label_name
psbA-trnK-UUU
feature_type
igs
region
LSC
length_bp
220
variable_sites
10
pi
0.0247
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.95
markerseek_score
63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA-trnK-UUU_p1 TACAGAAGCGACCCCATAGG CTAGTTCCGGGTTCGAATCC 55.0 55.0 58.6 57.8 291-307 bp 1.0 80.2
psbA-trnK-UUU_p2 TACAGAAGCGACCCCATAGG GAAAGCCGTGTGCAATGAAA 55.0 45.0 58.6 57.9 376-392 bp 1.0 79.7
psbA-trnK-UUU_p3 ATCCAGTTACAGAAGCGACC CTAGTTCCGGGTTCGAATCC 50.0 55.0 57.3 57.8 298-314 bp 1.0 79.5
psbA-trnK-UUU_p4 ATCCAGTTACAGAAGCGACC GAAAGCCGTGTGCAATGAAA 50.0 45.0 57.3 57.9 383-399 bp 1.0 78.9
psbA-trnK-UUU_p5 AGTTACAGAAGCGACCCCAT GAAAGCCGTGTGCAATGAAA 50.0 45.0 58.7 57.9 379-395 bp 1.0 78.5