Hotspot

rpl32

SSC · 133978-134145 · 168 bp

Back to Inga

Pi 0.519
MarkerSeek score 77.80
Primer available yes
Label rpl32

Feature Metadata

label_name
rpl32
feature_type
gene
region
SSC
length_bp
168
variable_sites
81
pi
0.519
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32_p1 TTAACGACCAAGTAAGCGGG AGCGTGTCTACCGATTTCAC 50.0 50.0 57.9 58.0 1036 bp 0.5 59.3
rpl32_p2 TTAACGACCAAGTAAGCGGG CAGCGTGTCTACCGATTTCA 50.0 50.0 57.9 58.0 1037 bp 0.5 59.3
rpl32_p3 TTAACGACCAAGTAAGCGGG TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.9 57.9 1046 bp 0.5 58.3
rpl32_p4 TTAACGACCAAGTAAGCGGG CCTAAGAGCAGCGTGTCTAC 50.0 55.0 57.9 57.8 1045 bp 0.5 57.8
rpl32_p5 TTAACGACCAAGTAAGCGGG CTAAGAGCAGCGTGTCTACC 50.0 55.0 57.9 57.8 1044 bp 0.5 57.8